Our research group mainly focuses on the development of spectroscopic probes and their bioanalytical applications. In the past 20 years, we proposed many new strategies for designing excellent spectroscopic probes, and developed a series of spectroscopic probes for various analytes by utilizing specific chemical reactions. These include: water-soluble polymeric chromogenic reagents for metal ions; triazine-based spectroscopic probes for N-terminal labeling and local structural analyses of proteins; spectroscopic probes with cleavable active bonds for reactive oxygen species and enzymes; highly selective spectroscopic sensing and imaging systems for amino acids, intracellular pH, hydrogen sulfide, etc.